Laboratory Tests - Immonology Department
Laboratory Tests - Immonology Department
The Immunology Laboratory of the NOVA Medical School|Faculdade de Ciências Médicas – Universidade NOVA de Lisboa has come to establish itself as a center for the provision of services to the community in the scope of primary immunodeficiency’s diagnostic.
For this purpose, we have validated testing protocols in the field of flow cytometry and Immunochemistry (set forth below), which represent an added value for the clinical evaluation of patients with suspicion of immunodeficiency.
Flow Cytometry
- Cytotoxic Activity of NK cells*
- Evaluation of the expression of membrane activation markers, after TLR stimulation
- Evaluation of the expression MHC I molecules (HLA ABC and β2microglobuline)
- Evaluation of the expression of Perforin
- Evaluation of the expression of membrane activation markers, after TLR stimulation
- Neutrophil phagocytic capacity
- Neutrophil oxidative capacity
- Characterization of the repertoire of TCR beta chain variable regions families
- Quantification of memory B cells
- Naive and memory T cells (CD4 or CD8)
- Evaluation of the expression of intracellular cytokines in lymphocytes, after stimulation
- Evaluation of the expression of intracellular cytokines in monocytes, after stimulation
- Basophil degranulation test
- CD40 expression
- PNH – identification and quantification in erythrocytes
- PNH – identification and quantification in leucocytes
- Primary Immunodeficiencies – initial study
- Evaluation of the expression of CD40 ligand, after stimulation
- Evaluation of the expression of membrane activation markers, after stimulation
- Evaluation of the expression of adhesion molecules
- Lymphocyte subsets T, B and NK
- T Lymphocyte subsets (CD4 and CD8) and leucocyte subsets in biologic fluids and lavages
- T Lymphocyte subsets (T cell receptor αβ and γδ)
- Quantitication of αβ+ CD4- CD8- T cells (ALPS)
- Evaluation of the expression of IL12 receptor, after stimulation
- Evaluation of the expression of Interferon gamma receptor
- T Lymphocyte subsets – Regulatory T cells with CD127
- T Lymphocyte subsets – Regulatory T cells with Fox p3
Immunochemistry
- IgE antibodies specific for isolated antigens and antigen mixes
- IgG antibodies specific for isolated antigens and antigen mixes
- Evaluation of the proliferative capacity in response to Antigens
- Evaluation of the proliferative capacity in response to Mitogens
- Evaluation of the IL12/IFN-γ axe
- Quantification of serum/plasma cytokines
- Hemolytic activity of complement – alternate pathway AH50
- Complement Factors (C1q, C2, C5, C6, C7, C8, C9, Properdin, B, D, H, I)
- Immunization to protein or polysaccharide antigens
- Circulating Immunecomplexes
- Immunoglobulin D
- Immunoglobulin E
- Inhibitor of esterase C’1
- Cationic Protein of eosinophils
- Immunoglobulin A Subclasses
- Immunoglobulin G Subclasses
- Triptase
